Knockout Mouse Catalog | Cyagen APAC
  1. Home
  2. Community
  3. Promotions
  4. Ready-to-Use Research Mouse Models

Immunotherapeutics, as a treatment of human disease, has brought benefits to both patients and commercial success to many drug developers. However, determining the best experimental immunotherapy model is still a major obstacle toward answering questions like how to optimize the immuno-oncology agent to finally developing better treatments.

Based on the complexity of human disease, the selection of the experimental mice is the key to the study. Combining the in-depth knowledge of each model immunological features and detailed model construction strategy according to specific requirements results in highly human-relevant models for progressing the research.

Intending to help researchers quickly obtain valuable research animal models for human disease studies, Cyagen has established its drug development and screening platform through the combination of introduction and self-development in high-quality control - to serve research in cancer, immunity, endocrinology, cardiovascular disease (CVD), neurology, and infectious diseases. We offer various ready-to-use research mouse models and keep developing models and services for drug development and efficacy evaluation is now a high priority in our current R&D activities.

Please complete the form to access Validation Data or order the model now >>

Congenital immunodeficient mice are the most widely used in scientific research studies, they are usually applied as laboratory animals for tumor models. Common examples of congenitally immunodeficient backgrounds include Nude mice, severe combined immunodeficient (SCID) mice, NOD Scid mice, among others. Among these, severe combined immunodeficient (SCID) mice are widely used in the construction of humanized mice due to their deficiency of T, B and NK immune cells.

Here Cyagen providing immunodeficient mouse models for your developing and testing immunotherapeutic strategies:

Category Strain Name Background Order
Immunodeficient Mice NOD-Scid NOD
C-NKG NOD/Shi-Scid
BRGSF BALB/c
BRGSF-HLA A2 /
Mice with Human Immune System BRGSF-HIS /

Compared with nude mice, SCID mice and NOD Scid, the Scid mouse - BRGSF is more easily to implanting into human tissues.
Here below are the comparisons of commonly-used Immunodeficient mice:

Mouse Strain Deficiency Advantages Disadvantages Applications
C-NKG No functional T, B and NK cells
Decreased complement activity
Impaired functions of macrophages and dendritic cells
No T and B cell leakage with age
Not well characterized.
CDX and PDX
Efficacy and safety assessment of cellular immunotherapy
Generation of humanized mouse models (e.g. using human PBMC or HSC to rebuild human immune system in mice)
GvHD
Immuno-oncology
Hematopoiesis and stem cells
BRGSF No functional T, B and NK cells
The most immunodeficient mouse models on the current market
Highly compatible with various sources of cell line-derived xenografts (CDXs) and patient-derived xenografts (PDXs)
Suitable for evaluating the therapeutical effect of radiotherapy
Powerful tool for complement-dependent cytotoxicity (CDC) studies
Not well characterized.
CDX and PDX (e.g., solid tumor and hematoma research)
Efficacy and safety assessment of cellular immunotherapy (e.g., CAR-T)
Vaccine development (e.g., HIV)
Myeloid compartment development (e.g., monocytes/macrophages)
Construction of mouse models with reconstituted human immune systems (e.g. BRGSF-HIS)
NOD-SCID No functional T and B cells, NK cells impaired Well-characterized. Low NK cell activity.
Very low leakiness with age.
High incidence of lymphomas.
Radiosensitive.
Higher levels of engraftment of PBMC and
hematopoietic stem cells compared with the SCID strain.
SCID (scid) No functional T and B cells Better engraftment of allogeneic and xenogeneic tumor cells and tissues than in Nude strain. Functional NK cells. Spontaneous lymphomas. Transplantation of murine and human (xenogeneic) tumors for imaging, metastasis and new therapies studies. Low levels of engraftment of human PBMC and fetal hematopoietic tissues.
>> Click here to learn more about our immunodeficient mice.

Since the current common animal models cannot truly simulate the tumor microenvironment (TME) of the patient with cancer, which greatly limits their applications in the area of translational Immunotherapy. Therefore, there is an urgent need to establish animal models with reconstituted human immune systems for relevant translational research. Mouse models with reconstituted human immune systems refer to immunodeficient mouse models engrafted with human hematopoietic stem cells (HSCs), lymphocytes or tissues to reconstitute a functional human immune system. These models can be used for tumor growth study in the environment of the human immune system, and anti-tumor therapy assessment, especially the efficacy of immunotherapy and related mechanisms.

>> Click here to learn more about BRGSF-HIS mice.

Immune checkpoints (ICPs) are regulators of immune activation. They help the immune system to maintain an optimal balance between autoimmune disease prevention and tumor elimination. Immune checkpoint inhibitors are antibody drugs developed for the corresponding immune checkpoints, which mainly reactivate the anti-tumor immune response by blocking co-suppressive T cell signaling. Although the amino acid sequence homology between the immune checkpoint genes of ordinary mice and the corresponding human genes is generally about 60%, antibodies that recognize human immune checkpoint proteins cannot identify the corresponding proteins in mice under normal circumstances - which may be solved with the humanization of mouse immune checkpoint genes.

Here Cyagen is providing humanized immune checkpoint mouse models for your developing and testing immunotherapeutic strategies:

Category Strain Name Background Order
Single-targeted Immune Checkpoint Mice hPD-1 C57BL/6N
hCTLA-4 C57BL/6N
hGITR C57BL/6N
hVISTA C57BL/6N
hOX40 C57BL/6N
hCD28 C57BL/6N
hCD39 C57BL/6N
BALB/c
Double-targeted Immune Checkpoint Mice hPD-1 & hVISTA C57BL/6N
hPD-1 & hCTLA-4 C57BL/6N
hCTLA-4/hLAG3 C57BL/6N
hPD-1/hTIM3 C57BL/6N
Key Features of Humanized Immune Checkpoint (ICP) Mice
  • Evaluating the effectiveness of drugs targeting human immune checkpoints efficiently
  • No need to develop a surrogate antibody
  • It can be used for the construction of a syngeneic mouse model
  • Compared with PBMC/HSC humanized mouse model, it greatly reduces costs, saves research resources, and efficiently obtains reliable experimental data.
Applications of Humanized Immune Checkpoint (ICP) Mice

The humanized immune checkpoint mouse enables the in vivo efficacy and safety assessment of fully immunocompetent mice:

  • ● Use with the mouse (humanized) tumor cell lines
    e.g. Humanized tumor cell line MC38-hPD-L1 - Use hPD-1 mouse model together with cell line MC38-hPD-L1 as a syngeneic tumor model for testing the efficacy of combination therapies targeting human PD-1 and human PD-L1.
  • ● Cross with humanized immune checkpoint mice
    e.g. hPD-1/hVISTA model - enables assessment of both compounds targeting human PD-1 and/or human VISTA in immunocompetent mice.
>> Click here to learn more about our humanized checkpoint mice.

As the world's leading provider of genetically engineered mouse and rat models, Cyagen’s drug research and development product line can serve drug discovery and screening in the fields of cancer, immunity, endocrinology, cardiovasology, neurology, infectious diseases, and more. Now we have opened a variety of Humanized Mouse Models to domestic scientific researchers, CRO institutions, and pharmaceuticals in accelerating research:

● Inflammation & Allergy Humanized Mice
Category Strain Name Background Order
Inflammation & Allergy Humanized Mice hCD4 C57BL/6J
hIgE/hFcεR1 BALB/c
hTNF-α C57BL/6J
>> Click here to learn more about inflammation & allergy humanized mice.
● Humanized Mice for PK/PD Research
Category Strain Name Background Order
Humanized Mice for PK/PD Research hFcRn/hSA - AlbuMusTM C57BL/6N
>> Click here to learn more about hFcRn/hSA - AlbuMusTM mice.
● Reporter Mice
Category Strain Name Background Order
Reporter Mice IL-4-IRES-eGFP (4get) BALB/c
Foxp3-IRES-mRFP (FIR) C57BL/6J
hGITR/Foxp3 C57BL/6N × C57BL/6J
>> Click here to learn more about reporter mice.
Model Resources

● Digital Resources:

Mouse Models for
Drug Screening & Assessment
(brochure)
Cyagen Animal Models
Catalog - 2021
(brochure)

 

● Technical Bulletin:

About Cyagen

In under 15 years since its founding, Cyagen has become a leading provider of custom mouse and rat models – delivering over 78,400 models to researchers worldwide and receiving over 4,800 peer-reviewed citations. From strategy design through to delivery of research-ready custom models, Cyagen offers complete outsourcing for all your animal model needs. We can create any custom mouse or rat model with guaranteed genotype validation, even offering phenotypic characterization and other downstream services required to your needs on neurodegenerative disease research.

 

Cyagen AI Knockout Mouse Model eBank

Over 16,000 knockout (KO)/conditional knockout (cKO) mouse models are available from the Cyagen AI Knockout Mouse Model eBank with the following advantages compared to other mouse model repositories:

  • Reduced Risk: Validated genome, guaranteed models
  • Skip Backcrossing: 100% pure C57BL/6 background
  • Fast Turnaround: 3,000 live strains, delivered as fast as 2 weeks
Search for your gene of interest >>

 

Efficient and intelligent Model Customization Platform
Cyagen's TurboKnockout® Gene Targeting service eliminates two generations of breeding, providing founder mice in as fast as 6 months. CRISPR-based Rapid Knockout services enable up to 500kb knockout (KO) and up to 15kb knockin (KI).
Drug Screening and Assessment Mouse Model Platform
We can provide genetically modified tumor mouse and rat models, immunodeficient mice, syngeneic model, human tumor cell line xenotransplantation (CDX) model, mice with reconstituted human immune system, immune checkpoint (ICP) humanized mice, models for metabolic disease and drug efficacy evaluations - including obesity, atherosclerosis, diabetes, non-alcoholic fatty liver disease (NAFLD), and more.
One-Stop Small Animal Phenotype Analysis Platform
Cyagen's one-stop small animal phenotype analysis platform can provide standardized phenotyping services covering cardiovascular, tumor, immunity, metabolism, nerve, and other fields.
Custom Cell Line Services
Cyagen can provide various types of custom cell line services such as vector construction, virus packaging and over expression, interference, gene knockout cell line, point mutation cell line and gene knockin cell line.

 

Partnership
>> Cyagen and genOway have joined forces to form a long-term strategic partnership in the Asia-Pacific region

With this agreement, Cyagen obtains rights to distribute genOway’s preclinical humanized immune checkpoint and immunodeficient BRGSF mouse models in the Asia-Pacific region.

 

*If you require any further information, please contact us.