The humanized LIGHT mouse model (hLIGHT) is developed by knockin at the mouse LIGHT locus, and expresses a chimeric LIGHT with a human extracellular, human transmembrane and murine intracellular domain.
The hLIGHT mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint (ICP) VISTA in fully immunocompetent mice.
Figure 1. hLIGHT is expressed on activated splenic T cells. Expression of human LIGHT (hLIGHT) on splenic Teff and CD8 T cells activated with αCD3/αCD28 in presence of rmIL-2 for 24h. hLIGHT is expressed on spleen effector T cells and CD8 T cells, but not on splenic T cells of WT mice.
Figure 2. hLIGHT stimulation in hLIGHT models increases T cell activation. T cells from wild-type and hLIGHT mice were stained with CTV and activated with coated amCD3, with or without recombinant mouse (rm) or human LIGHT, and with or without a-human LIGHT. At day 4 proliferation was measured by FACS (A), and IL-2 production was measured in the supernatant (B).
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|Category||Strain Name||Background||Order Amount
|Single-targeted ICP Mice||hLIGHT||/||/||/|
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With more than 15 years’ experience in genetic engineering, Cyagen offers a complete range of products and services to support the advancement of human disease research. Cyagen is committed to enabling the development of therapeutics for human diseases by providing one-stop research solutions – from custom rodent model generation and breeding, through therapeutic viral packaging and injection, and phenotype analysis to study disease mechanisms, target validation, drug screening and more.
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