Knockout Mouse Catalog | Cyagen APAC

hLIGHT Mouse

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Model Description

Background: C57BL/6N

Construction Strategy:
The humanized LIGHT mouse model (hLIGHT) is developed by knockin at the mouse LIGHT locus, and expresses a chimeric LIGHT with a human extracellular, human transmembrane and murine intracellular domain.



  • hLIGHT expression displays a physiological regulation and expression pattern
  • Fully functional mouse immune system
  • Lack of expression of the murine target gene, thus avoiding cross-reactivity


Research and Application:

The hLIGHT mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint (ICP) VISTA in fully immunocompetent mice.



  • LIGHT expression analysis
Model Validation

Figure 1. hLIGHT is expressed on activated splenic T cells. Expression of human LIGHT (hLIGHT) on splenic Teff and CD8 T cells activated with αCD3/αCD28 in presence of rmIL-2 for 24h. hLIGHT is expressed on spleen effector T cells and CD8 T cells, but not on splenic T cells of WT mice.


  • LIGHT function analysis
Model Validation

Figure 2. hLIGHT stimulation in hLIGHT models increases T cell activation. T cells from wild-type and hLIGHT mice were stained with CTV and activated with coated amCD3, with or without recombinant mouse (rm) or human LIGHT, and with or without a-human LIGHT. At day 4 proliferation was measured by FACS (A), and IL-2 production was measured in the supernatant (B).


*For more validation data please contact us.

Category Strain Name Background Order Amount
List Price
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Single-targeted ICP Mice hLIGHT / / /

Price Availability

  • Sex: Female
  • Age: 3 to 8 weeks
  • Customers in Asia Pacific region, excluding Japan.

*If you would like to order male mice, Please contact us.

Digital Resources:

hLIGHT Mouse
Mouse Models for
Drug Screening & Assessment
Cyagen Animal Models
Catalog - 2021

Why work with Cyagen?

With more than 15 years’ experience in genetic engineering, Cyagen offers a complete range of products and services to support the advancement of human disease research. Cyagen is committed to enabling the development of therapeutics for human diseases by providing one-stop research solutions – from custom rodent model generation and breeding, through therapeutic viral packaging and injection, and phenotype analysis to study disease mechanisms, target validation, drug screening and more.

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,800+ citations in SCI journals for our services
  • 3,000+ university and company partners
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