Knockout Mouse Catalog | Cyagen APAC

hIgE/hFcεR1 Mouse

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Model Description

Background: BALB/c

Construction Strategy:
Chimeric IgE/FcεR1 complex with humanized IgE/FcεR1 interactive domains (i.e., IgE Fc region and FcεR1 α-chain), and murine IgE Fab region and FcεR1 β- and γ-chains]

hIgE/hFcεR1 Mouse

 

Features:

  • Human-like cellular distribution (i.e., mast cells, basophils, monocytes/DCs, Langerhans cells, and eosinophils) of the FcεR1 receptor due to the presence of a minimal human promoter that drives its expression
  • Physiological regulation and expression of the chimeric IgE/FcεR1 complex
  • Human IgE immunoglobulin and no mouse IgE immunoglobulin (Fig. 1B)
  • Human FcεRI receptor and no mouse FcεRI receptor (Fig. 1A)

 

Applications:

The humanized IgE/FcεR1 mouse model has been successfully used to screen for innovative therapeutics for allergy(e.g., allergic rhinitis, atopic dermatitis, food allergies, chronic urticaria), asthma and other IgE-mediated diseases (Eosinophilic esophagitis and inflammatory bowel disease)s, and improve the translatability and predictability of IgE-mediated reactions.

 

  • FcεR1 expression analysis
Model Validation

Figure 1. Expression of hFcεR1 in humanized IgE/FcεR1 mouse cells.Left panel: Expression of hFcεR1 α-chain from bone marrow-derived cultured mast cells (BMMCs) in presence of murine IL-3, stem cell factor (SCF) and IL-6 (8 weeks treatment). Right panel: Expression of hFcεR1 on murine eosinophils in peripheral blood upon intravenous injection of eotaxin (2.4 nmol/kg)

 

  • IgE expression analysis
Model Validation

Figure 2. Analysis of human FcεR1 and human IgE expression in double humanized IgE/FcεR1 mouse cells. a) Expression of hFcεR1-α chain from bone marrow-derived mast cells cultured in presence of murine IL3, SCF, and IL6 for 8 weeks. b) Human IgE in serum of mice sensitized and challenged with ovalbumin. (black column: treated; white column: untreated)

 

  • FcεR1 and IgE function analysis
Model Validation

Figure 3. The functional data of the humanized IgE/FcεR1 mouse model. (Left) After binding to hFcεR1, IgE triggers the degranulation of mast cells. (Right) Mast cells respond to hFcεR1, but do not respond to mFcεR1 cross-linking (XL).

Conclusion: Mast cells from the humanized model bind human IgE and degranulate upon cross-linking. This is specific and not restricted to given antigen. This set of data validates the functionality of the IgE high-affinity receptor. It binds human IgE and triggers degranulation upon cross-linking.

 

Model Validation

Figure 4. Functional data on double humanized IgE/FcεRI model: Inhibition of mast cell degranulation by anti-IgE monoclonal Ab. Mast cells were sensitized overnight with human IgE in presence of indicated biologics. The cells were washed and stimulated with anti-IgE. β-hexosaminidase activity in cell supernatant was determined as a percentage of total β-hexosaminidase activity in cell lysates.

Conclusion: Anti-IgE Ab, but not the isotype control, suppresses hFcεR1-mediated degranulation in a dose-dependent manner.

 

*For more validation data please contact us.

Category Strain Name Background Order Amount
(head)
List Price
(USD/head)
Order Now
Inflammation &
Allergy Humanized Mice
hIgE + FcεR1 BALB/c 1~50 $619
51-199 $559
>200 $509

Price Availability

  • Sex: Female
  • Age: 3 to 8 weeks
  • Customers in Asia Pacific region, excluding Japan.

*If you would like to order male mice, Please contact us.

Digital Resources:

hIgE/hFcεR1 Mouse
(poster)
Download
Mouse Models for
Drug Screening & Assessment
(brochure)
Download
Cyagen Animal Models
Catalog - 2021
(brochure)
Download

Why work with Cyagen?

With more than 15 years’ experience in genetic engineering, Cyagen offers a complete range of products and services to support the advancement of human disease research. Cyagen is committed to enabling the development of therapeutics for human diseases by providing one-stop research solutions – from custom rodent model generation and breeding, through therapeutic viral packaging and injection, and phenotype analysis to study disease mechanisms, target validation, drug screening and more.

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,800+ citations in SCI journals for our services
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured

*If you require any further information, please contact us.

 

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