Knockout Mouse Catalog | Cyagen APAC

hCD4 Mouse

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Model Description

Background: C57BL/6J

 

Features:

  • No physiological deregulation: Unaltered immune cell distribution, and T helper cell activity
  • Lack of expression of the corresponding murine gene

 

Application:

The human CD4 mouse model allows in vivo efficacy and safety assessment of compounds targeting human CD4, and is generally used in fields of inflammation, autoimmune diseases, immuno-oncology and HIV infection.

 

  • CD4 expression analysis
Model Validation

Figure 1. Human CD4 replaces murine CD4. Homozygous CD4 humanized mice display human-CD4 expression in PBMC. No murine CD4 expression is observed. Blood samples from wild-type and homozygous CD4 humanized mice stained with a combination of mAbs (anti-murine CD4, anti-human CD4) and analyzed by flow cytometry.

 

  • Immune cells distribution analysis
Model Validation

Figure 2. Unaltered immune cell distribution. Homozygous CD4 humanized mice display same frequencies of B cells, NK cells and T cells in spleen as the wildtype mice. Splenocytes are stained with different mAb combinations to assess frequencies of lymphocyte subsets measured by flow cytometry. Grey bars represent values for wild-type mice and hatched bars represent values for CD4 humanized mice.

 

  • Cytokines expression analysis
Model Validation

Figure 3. Conserved cytokine expression profile. Homozygous CD4 humanized mice display similar cytokine expression after ConA stimulation. IL-2, IFN-γ and IL-4 concentrations in culture supernatants of mouse splenocytes stimulated with Con A are measured by using a 3-Plex Luminex kit. Black bars represent values for wild-type mice and hatched bars represent data for humanized CD4 mice.

 

  • Ig production analysis
Model Validation

Figure 4. Similar Ig production. Homozygous CD4 humanized mice display similar anti-KLH isotyping to wild-type mice. Anti-KLH specific IgG1, and IgG2b polyclonal antibodies are assessed by sandwich ELISA in the serum of mice at day 28 after immunization with KLH and CFA. Serum samples from wild-type (WT) or homozygous CD4 humanized mice (HO CD4) were tested at different dilutions plotted against OD values.

 

*For more validation data please contact us.

Category Strain Name Background Order Amount
(head)
List Price
(USD/head)
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Inflammation & Allergy Humanized Mice hCD4 / / /

Price Availability

  • Sex: Female
  • Age: 3 to 8 weeks
  • Customers in Asia Pacific region, excluding Japan.

*If you would like to order male mice, Please contact us.

Digital Resources:

hCD4 Mouse
(poster)
Download
Mouse Models for
Drug Screening & Assessment
(brochure)
Download
Cyagen Animal Models
Catalog - 2021
(brochure)
Download

Why work with Cyagen?

With more than 15 years’ experience in genetic engineering, Cyagen offers a complete range of products and services to support the advancement of human disease research. Cyagen is committed to enabling the development of therapeutics for human diseases by providing one-stop research solutions – from custom rodent model generation and breeding, through therapeutic viral packaging and injection, and phenotype analysis to study disease mechanisms, target validation, drug screening and more.

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,800+ citations in SCI journals for our services
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured

*If you require any further information, please contact us.

 

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