As researchers investigate the implications of ACE2 in COVID-19 pathology, different animal models may be needed to explore the mechanisms of ACE2 in its entirety. There are multiple approaches to developing humanized ACE2 (hACE2) models, including ROSA26 conditional knockin (cKI) or endogenous replacement, which yield different proteins. Given the early stages of most ACE2 research performed in animal models, there has yet to be sufficient evidence to determine the explicit advantages of either humanized model generation method.

Despite these challenges, using a variety of animal models can serve to elucidate the function of ACE2 in COVID-19 onset. By comparing results across both hACE2 models and background strains, we can understand how each model serves to recapitulate both the virology of SARS-CoV-2 and pathology of COVID-19 in humans. To support this approach, Cyagen give special-offer prices to those ordering mice from across ACE2 model generation methods and/or strain backgrounds - Get any hACE2 or ACE2 KO models in either C57BL/6J or BALB/c strain backgrounds. Additionally, we are excited to be able to provide several technical reports about the progress on these ACE2 models on your request.

Promotion Period: July 1st 2020 - August 31st 2020     Eligibility: End clients in South Korea only.

For humanized models, knockout models, Rosa26 knockin mouse models of ACE2 and DPP4 on C57BL/6 mouse strains, the exclusive sales prices are as below:

Note:

• 1. In addition to the above-listed models, Cyagen is also prioritizing inquiries on other COVID-19 models, including DPP4, APN, TMPRSS2 and CD147 gene targets. 
• 2. Cyagen can also offer mouse models in BALB/c background, please contact us for further information.

Genetic Overview

• ● Background: C57BL/6J Strain
• ● Gene targeted: Ace2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [Mus musculus (house mouse)]
• ● Gene ID: 70008
• ● Mutation Type: CRISPR/Cas9 mediated gene knockout
• ● Description: The region from exon 3 to exon 18 of mouse Ace2 was selected as the target site. Exon 3-18 covers 87.91% of the coding region.
• ● Mutation Type: CRISPR/Cas9 mediated gene knockin
• ● Description: the“CAG promoter-loxP-PGK-Neo-6*SV40 pA-loxP-Kozak-Human ACE2 CDS-rBG pA” cassette will be cloned into intron 1 of ROSA26.
• ● Mutation Type: CRISPR/Cas9 mediated gene knockin
• ● Description: the region from aa.18 to aa.805 of mouse Ace2 will be replaced with the region from aa.18 to aa.805 of human ACE2. The murine signal peptide (aa.1~17) will be remained.

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