Knockout Mouse Catalog | Cyagen APAC

PiggyBac Transgenic Mice

Piggybac Transgenic Mice Services
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Mechanism of piggyBac transposition

● Transposons (or transposable element) – small piece of DNA that inserts itself into another place in the genome.

● PiggyBac is a mobile genetic element that efficiently transposes between vectors and chromosomes via “Cut & Paste” mechanism.

● Pbase recognizes ITRs on vector and moves transgene and integrates into TTAA chromosomal sites.

bac transgenic mouse services

 

Workflow of PiggyBac Transgenic Mouse Projects

Each full PiggyBac transgenic mouse project is divided into the following phases:

bac transgenic mouse model services

1. PiggyBac transgenic strategy design

  • Design a PiggyBac transgenic strategy that meets your research needs.
  • Develop a PCR genotyping strategy for PiggyBac transgenic founder screening.

 

2. PiggyBac transgenic vector construction

  • Construct PiggyBac plasmid-based transgenic vectors.
  • Confirm the constructed PiggyBac-based transgenic vectors by restriction digest and sequencing, as needed.

 

3. PiggyBac transgenic pronuclear injection

  • Inject the verified PiggyBac transgenic plasmid into the pronucleus of fertilized eggs.
  • Implant injected eggs into surrogate mothers to obtain offspring.

 

4. PiggyBac transgenic mouse screening

  • Genotype pups by PCR to identify those carrying the PiggyBac transgene.

 

 Guarantee

Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.

Why work with Cyagen?

  • 14+ years' experience in custom animal model generation
  • 78,000+ animal models generated and delivered worldwide
  • 4,700+ citations in SCI journals for our services
  • 3,000+ university and company partners
  • 100% money-back guarantee option available for most services
  • AAALAC-Accredited and OLAW Assured
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