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● Transposons (or transposable element) – small piece of DNA that inserts itself into another place in the genome.
● PiggyBac is a mobile genetic element that efficiently transposes between vectors and chromosomes via “Cut & Paste” mechanism.
● Pbase recognizes ITRs on vector and moves transgene and integrates into TTAA chromosomal sites.
Each full PiggyBac transgenic mouse project is divided into the following phases:
1. PiggyBac transgenic strategy design
2. PiggyBac transgenic vector construction
3. PiggyBac transgenic pronuclear injection
4. PiggyBac transgenic mouse screening
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.