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“Among several commercial services who have claimed to be specialized to generate mouse models with genetic modifications, I recognize that Cyagen is one of the best. We are looking forward to having more cooperation with them.”

Korea Brain Research Institute (KBRI)

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Knockout Cell Lines

Name: Knockout Cell Lines
Price: None USD

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Using CRISPR/Cas9 technology, we disable the gene of interest by the removal of large fragments of the gene instead of the introduction of a frameshift mutation, which will make sure the complete loss of gene function. Cyagen is the world`s leading provider of custom genome editing services. Leverage our experts and optimized CRISPR/Cas9 cell line modeling service platform CRISPR-Pro for a hassle-free research. CRISPR-Pro enables large fragments excision or correct mutations in various cell lines.

◆ Workflow

◆ Variety of cell lines

Cancer cells, hard-to-transfect cells, stem cells and more.

◆ Advantages of large fragment knockout

Our CRISPR-Pro technology can bring paired gRNAs by which large fragment deletion can be obtained. Compared with frameshift mutations, the removal of large fragments of the gene is more efficient and straight-forward.

Comparison	Large fragment KO		Frameshift mutation
	Plasmid electroporation	Lentivirus transduction	Plasmid electroporation	Lentivirus transduction
Outcomes	Complete deletion of large fragment enables inactivation for all transcripts, much likely including unknown transcripts as well.		Transcripts from downstream and some unknown transcripts may not be affected.
Deletion Limit	Large fragments		One or several base pairs
Identification	Easy by PCR assays		Sequencing needed
If Virus Packaging Needed	No	Yes	No	Yes
Turnaround	2~3 months	3~5 months	2~3 months	3~5 months

◆ Why work with Cyagen?

Guaranteed: 100% money back guarantee
Reliable: Complete loss of gene function
Short timeline: As fast as 2 months
Substantial experience: Over 13 yearsof experience with thousands of gene editing projects delivered.
Optimized technology: By using optimized CRISPR/Cas9 technology CRISPR-Pro, the removal of large fragments of the gene can be achieved.
Customer citations: Over 2,400 publications citing our services and products.

◆ Inquiries and quote requests

Request a quote now. Alternatively, you can always email service-apac@cyagen.com to inquire about our services or obtain a quote for your project.

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