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information and estimated timeframe to
your project needs.
In addition to the design and custom generation of genetically engineered mouse/rat models, Cyagen’s breeding service provides reliable, economical, and efficient breeding colonies of mice/rats to support your research needs; allowing you to focus more on your research goals and less on animal care, breeding and colony management issues.
We provide the following breeding services in our AAALAC-accredited, OLAW-assured SPF facility.For most transgenic projects, the deliverables are F0 founder animals. Given that the transgenes are randomly integrated into genome, and a small percentage of founder animals display some degree of mosaicism, there is a possibility that some F0 animals do not transmit the transgene to their offspring. Cyagen will breed founder animals to wt animals of matching background, and screen F1 animals with desired mutation(s). A minimum of 2 F1 animals per line will be generated and delivered.
| Service | Price | Turnaround Time | |
| Transgenic founder mice/rats breeding to F1 | mice | $1,450/first line, $1,000/per additional line | ~3 months |
| rats | $1,950/first line, $1,200/per additional line | ~3 months | |
Note: Large cohorts of F1 or F2 animals can be generated at additional cost.
1. Standard KO/KI mice breeding service
Cyagen will set up heterozygote x heterozygote breeding and screen pups by PCR for targeted mutation(s). Theoretically this breeding will produce 50% heterozygotes, 25% homozygotes and 25% wt animals. We will provide 2~4 homozygous mice per line. If no homozygotes are identified from a total of 30 pups screened, it is possible that the homozygous mutation may lead to embryonic lethality. If this is the case we will stop breeding and discuss the project with clients.
2. Conditional KO/KI mice breeding service
Cyagen can inter-cross flox/wt mice to generate flox/flox mice, and can further breed them with Cre deleter of your choice to produce tissue-specific KO/KO (or KI/KI) mice. We will deliver 2~4 flox/flox mice or 2~4 tissue-specific KO/KO (or KI/KI) mice.
3. Price and turnaround time
| Service | Price | Turnaround Time | |
| Standard KO/KI mice breeding service | $3,450 | 3~6 months | |
| Conditional KO/KI mice breeding service | flox/flox | $3,450 | 3~6 months |
| tissue-specific KO/KO and KI/KI | $6,450 | 5~9 months | |
Note: Large cohorts of homozygous animals can be generated at additional cost.
Although animals generated by CRISPR-mediated genome editing carry targeted mutation(s), the founder animals are different from animals generated by ES cell gene targeting approaches in that most of the founder animals display some degree of mosaicism. Cyagen offers founder animal breeding service which allows us to deliver germline transmission-confirmed heterozygous/homozygous animals to researchers.
We guarantee generation and delivery of a minimum of 3 heterozygotes. We can further inter-cross heterozygotes and deliver homozygous animals (a minimum of 2 per line). If no homozygotes are identified from a total of 30 pups screened, it is possible that the homozygous mutation may lead to embryonic lethality. If this is the case we will stop breeding and discuss the project with clients.
| Service | Price | Turnaround Time |
||
| Mice | Rats | |||
|
CRISPR-mediated genome editing mice/rats breeding |
heterozygous |
$1,450/first line, $1,000/per additional line |
$1,950/first line, $1,200/per additional line |
3~4 months |
| homozygous |
$2,450/first line, $1,500/per additional line |
$3,450/first line, $2,000/per additional line |
4~8 months | |
Note: Large cohorts of homozygous animals can be generated at additional cost.