We offer a one-stop solution for all your transgenic mouse embryos needs. You can contract us to perform your entire transgenic mouse embryo project from initial strategy design and DNA vector construction to transgenic mouse embryo delivery, or you can outsource specific phases of the project to us.
◆ Workflow of Transgenic Mouse Embryo Services
1. Transgenic strategy design
- Design a transgenic strategy that meets your research needs.
- Develop a PCR genotyping strategy for mouse embryos screening.
2. Transgenic vector construction
- Construct conventional plasmid-based transgenic vectors, or modify BACs by recombineering as needed for BAC-based transgenes (visit our DNA Vector Construction & BAC Modification Services page for details).
- Confirm plasmid or BAC modification by restriction digest and sequencing as needed.
3. Pronuclear injection
- Inject transgenic plasmid or BAC into the pronucleus of fertilized eggs.
- Implant injected eggs into surrogate mothers to obtain offspring.
4. Transgenic mouse embryos screening and LacZ staining
- Embryos harvested at the desired embryonic stage will be genotyped by PCR and lacZ stained to identify the ones carrying the desired transgene.
Cyagen offers the best guarantee in the industry – we will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.
Why work with Cyagen?
years' experience in custom animal model generation
animal models generated and delivered worldwide
citations in SCI journals for our services
university and company partners
● 100% money-back guarantee
option available for most services
● AAALAC-Accredited and OLAW Assured