CRISPR/Cas9-based technology. Cyagen’s CRISPR-based gene targeting services can generate custom mice and rats – including conventional knockout, conditional knockout, large-fragment knockin (LFKI), and humanization models - in as fast as 3 months.

Compared with other gene editing technologies, the key advantages of CRISPR/Cas9 are its high efficiency, rapid turnaround, low cost, convenient process, and successful application to different species. CRISPR/Cas9 significantly reduces turnaround time compared with traditional embryonic stem (ES) cell-mediated gene targeting due to its direct application in embryo. Our AI-based bioinformatics tools, such as AlphaKnockout, rapidly identify the optimal sequences for guide RNAs (gRNAs) to minimize off-target effect and guarantee the

Cyagen offers the best guarantee in the industry – we guarantee generation of constitutive knockout mice. We will fully refund the client’s service fee if animals with the specified genotype are not generated (except for genetic modifications severely affecting viability, morbidity, or fertility). Given the complexity of biological systems, a particular genetic modification may not result in the desired phenotype. As such, Cyagen's guarantee covers the creation of animals with the specified genotype, not a particular phenotypic outcome in terms of transcription, protein/RNA function, or organismal biology.