Knockout Mouse Catalog | Cyagen APAC

Knockin Mice

A Knockin mouse defines an animal model in which a gene sequence of interest is altered by on-for-one substitution with a transgene, or by adding gene sequences that are not found within the locus. Cyagen offers a one-stop solution for all your gene targeting mouse model needs. Our knockin services include conventional knockin mice, humanized mice, point mutation mice and ROSA26 knockin mice. You can contract us to perform your entire targeting project from initial strategy design and targeting vector construction to mutant animal delivery, or you can outsource specific phases of the project to us.
◆ Cyagen Knockin Mouse Model Solution

1. ESC-based Gene Targeting - TurboKnockout ®

- Conditional knockin mice

- Humanized mice

TurboKnockout ®, the newest ES-cell based gene targeting technology which eliminates two generations of breeding, allowing the generation of conditional knockin mouse models as fast as 6 months, 4-6 months shorter than the standard approaches.

Highlights of TurboKnockout ®:

  • Timeline comparable to nuclease-based genome editing (i.e., CRISPR, TALEN)
  • More reliable than nuclease mediated methods (e.g., conditional models, humanizations)
  • Guaranteed germline transmission
  • No off-target effects
  • Comparable cost to traditional ES cell methods

2. CRISPR/Cas-based Knockin Mice

Genome editing using CRISPR/Cas system can generate constitutive knockin founder animals in as fast as 3 months.

- Point mutation mice

Cyagen offers quick point mutation mouse model generation via CRISPR/Cas-mediated genome editing technology by co-injecting nuclease and oligo donors carrying the desired mutation and homology arms.

- ROSA26 knockin mice

Full control of your gene expression by targeting the best-studied permissive loci ROSA26.

- Knockin mice

Cyagen offers CRISPR-based knockin at any locus, and conditional knockin mice generation in as fast as 4 months, at highly competitive prices.



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