|Conditional Knockout Mice
|≥3 cKO Mice
|As fast as 3 months
Conditional knockout mice play a vital role in biomedical advancements across many research areas which is essential for understanding human diseases and accelerating drug development. Compared with conventional knockout mice, the precise temporal and spatial control of gene expression provided by cKO/floxed models yields greater experimental capabilities.
For a limited time, get your conditional knockout (cKO) mice for only $10,000 from our selection of Cyagen AI Knockout Mouse Model eBank. Take advantage of this promotion before it ends!
Over 16,000 KO and cKO Mouse Models | 100% Pure B6 Background | Delivered as Fast as 3 Months
Cyagen has generated a number of conditional knockout mouse models with the using of CRISPR technology and the Cre-Loxp system for Cre conditional knockout (cKO). In this case, gene Slc30a7 conditional knockout mouse line was created in the C57BL/6N background and subsequently crossed with the proper Cre animals. The strategy of gene Slc30a7 cKO mice was to select exon 4 and exon 5 as conditional knockout regions. CRISPR technology was used for insertion of two LoxP sites flanking the cKO region for conditional removal using Cre conditional knockout technology.
Diagram 1. The schematic describes the first stage in developing a conditional knockout mouse model using CRISPR to generate a floxed mouse.
Figure 1. PCR genotype screening of the floxed mice. Amplify 5'LoxP and 3'LoxP fragments by using two different PCR primers, candidate positive transformant genomic DNA as template, wild-type and water as control. PCR results showed four mice were identified as potential founders.
Figure 2. Chromatograms of 5'LoxP and 3'LoxP PCR fragments from the floxed mouse. Genomic DNA extracted from individual mice born from microinjection of the embryos were subjected to genotyping PCR. Sequencing results showed candidate positive mouse No. 51 has LoxP insertions.
Promotion for Downstream Breeding Service
To support researchers seeking to study homozygous knockout (KO) mouse models, we are making homozygous breeding upgrades available with any purchase from our Knockout Catalog Models. Place an order from the Knockout Catalog Model by May 31, 2022 to receive a discounted upgrade for homozygous deliverables - saving $2,450! Mention promotion code "HOMO22" when submitting quote request.