Knockout Mouse Catalog | Cyagen APAC
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Only $8,000! Get Your Conditional Knockout Mice
Only $8,000
Get Your Conditional Knocckout Mice

Conditional knockout mice play a vital role in biomedical advancements across many research areas which is essential for understanding human diseases and accelerating drug development. Compared with conventional knockout mice, the precise temporal and spatial control of gene expression provided by cKO/floxed models yields greater experimental capabilities.

For a limited time, get your conditional knockout (cKO) mice for only $8,000 from our selection of Cyagen AI Knockout Mouse Model eBank. Take advantage of this promotion before it ends!

Conditional Knockout Mice
Project Type Deliverables Turnaround Time Promotion Price
(USD/Strain)
Model Ordering
Conditional Knockout Mice
(cKO)
≥3 cKO Mice As fast as 3 months $8,000
● Promotion Period: May 15 - July 15, 2022
● Eligibility: End clients in Singapore. Contact us for availability in other regions.
Cyagen Knockout Catalog Models

Over 16,000 KO and cKO Mouse Models | 100% Pure B6 Background | Delivered as Fast as 3 Months

Advantages of Cyagen AI Knockout Mouse Model
  • Validated Models: Our catalog features gene knockout mice that have been strictly analyzed with PCR to ensure 100% positive knockout per the gene targeting strategy.
  • Professional Technical Support: Competitor price matching & 24/7 technical support
  • AAALAC-Accredited and OLAW Assured
  • Deliverables: As fast as 3 months
  • Reduced paperwork: Inquire online to place an order, one click to get detailed information and modeling guidance, backed by a powerful database.
  • 100% Pure B6 background Eliminates need for backcrossing – most repositories have mixed backgrounds
  • Inexpensive: Cost of catalog lines cannot be beaten by any model generation approach
Case Study

Cyagen has generated a number of conditional knockout mouse models with the using of CRISPR technology and the Cre-Loxp system for Cre conditional knockout (cKO). In this case, gene Slc30a7 conditional knockout mouse line was created in the C57BL/6N background and subsequently crossed with the proper Cre animals. The strategy of gene Slc30a7 cKO mice was to select exon 4 and exon 5 as conditional knockout regions. CRISPR technology was used for insertion of two LoxP sites flanking the cKO region for conditional removal using Cre conditional knockout technology.

Diagram 1. The schematic describes the first stage in developing a conditional knockout mouse model using CRISPR to generate a floxed mouse.

Figure 1. PCR genotype screening of the floxed mice. Amplify 5'LoxP and 3'LoxP fragments by using two different PCR primers, candidate positive transformant genomic DNA as template, wild-type and water as control. PCR results showed four mice were identified as potential founders.

Figure 2. Chromatograms of 5'LoxP and 3'LoxP PCR fragments from the floxed mouse. Genomic DNA extracted from individual mice born from microinjection of the embryos were subjected to genotyping PCR. Sequencing results showed candidate positive mouse No. 51 has LoxP insertions.

FAQs
Where do your mice come from?
Our main source of mice is Charles River Laboratory. Currently, only mice from Charles River laboratory, Jackson Laboratory and Taconic are allowed into our facility.
How long does it take from DNA microinjection to obtaining transgenic animals?
Typically, it takes about 8 weeks from receipt of DNA to shipment of first-generation mice: 1-2 weeks to complete the microinjection process, around 3 weeks for pups to be born, and 3-4 weeks before mice are weaned and shipped from our facility. The quality of the DNA preparation and the accuracy of the concentration detection can significantly affect the project turnaround, so we will request that DNA that does not meet our criteria be replaced. For those services requiring only transgenic embryos, the time can be as short as 4-5 weeks. However, if the number of injections needs to be increased, the project turnaround may be extended as appropriate.
What is your success rate in generating first-generation transgenic founders? Is there a guarantee?
The success rate of generating first-generation transgenic mice with plasmid DNA is approximately 3-4% (i.e., approximately 5-8 PCR-positive first-generation mice can be obtained for each injection of 200 zygotes). We promise to provide at least two PCR-positive first-generation transgenic mice. We guarantee at least two first-generation mice that carry some or all of the transgene, but we do not guarantee expression of the transgene or germline inheritance. If the transgene is embryonic lethal or the DNA provided by the customer does not meet the requirements, the customer needs to understand that they will bear the full cost of the microinjection. Due to the lower success rate of BAC, there is no guarantee on first-generation transgenic mice, however, customers can choose to add an additional round of injections to improve their chances of success with our discounted option.

Promotion for Downstream Breeding Service

To support researchers seeking to study homozygous knockout (KO) mouse models, we are making homozygous breeding upgrades available with any purchase from our Knockout Catalog Models. Place an order from the Knockout Catalog Model by May 31, 2022 to receive a discounted upgrade for homozygous deliverables - saving $2,450! Mention promotion code "HOMO22" when submitting quote request.