We have selected several high-impact factor (IF) publications which use conditional knockout (cKO), a.k.a. floxed, mouse models developed by Cyagen. The following publications demonstrate the use of conditional knockout mouse models across a range of human disease research fields, including immunology, cardiology, metabolomics, oncology and more.
| Research Applications | Gene Target(s) | Title | Journal Information |
|---|---|---|---|
| Immunology, Gut | Ddx58 | Commensal viruses maintain intestinal intraepithelial lymphocytes via noncanonical RIG-I signaling. | Nature Immunology PMID: 31636462 |
| Cell Biology, Cardiovascular | SAHH(Ahcy) | Inhibition of S-Adenosyl homocysteine Hydrolase Induces Endothelial Dysfunction via Epigenetic Regulation of p66shc-Mediated Oxidative Stress Pathway | Circulation DOI: 10.1161/circulationaha.118.036336 |
| Oncology | Trib3 | TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARa and Inhibition of p53-Mediated Senescence | Cancer Cell DOI: 10.1016/j.ccell.2017.04.006 |
| Cell Biology | CK2α′ | Small molecule induces mitochondrial fusion for neuroprotection via targeting CK2 without affecting its conventional kinase activity | Signal Transduct Target Ther. DOI: 10.1038/s41392-020-00447-6 |
| Immunology | NMBR | Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition | Nature Immunology DOI: 10.1038/s41590-020-0753-y |
| Immunology | Rab27a | Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses | Immunity DOI: 10.1016/j.immuni.2019.01.010 |
| Metabolism, Molecular biology | DDIT | DDIT3 Directs a Dual Mechanism to Balance Glycolysis and Oxidative Phosphorylation during Glutamine Deprivation | Advanced Science DOI: 10.1002/advs.202003732 |
| Gastroenterology | TMEM16A | Hepatocyte TMEM16A Deletion Retards NAFLD Progression by Ameliorating Hepatic Glucose Metabolic Disorder | Advanced Science DOI: 10.1002/advs.201903657 |
| Molecular Biology | Larp7 | LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice | Molecular Cell DOI: 10.1016/j.molcel.2020.01.002 |
| Metabolism, Liver | Crebzf | Hepatic CREBZF Couples Insulin to Lipogenesis by Inhibiting Insig activity and Contributes to Hepatic Steatosis in Diet-Induced Insulin-Resistant Mice | Hepatology DOI: 10.1002/hep.29926 |
Genetically engineered animals remain essential for gaining proper understanding of human disease mechanisms and play an important role in a variety of research areas. Since our founding in 2005, Cyagen has been cited in over 4,800 publications – all of which are available as a searchable list:
Cyagen's all-in-one innovative CRO service network platform can provide genetically engineered mouse models, ready-to-use models, and model supporting services for your demands on experimental animal models:
TurboKnockout® Gene Targeting Services
TurboKnockout® services can deliver conditional knockout (cKO/floxed) in as fast as 6 months. For TurboKnockout®-mediated conditional knockout (cKO/floxed) mice, it may be possible to breed founders directly to your tissue-specific Cre mice.
Advantages of TurboKnockout®
● Freedom to Operate: Technology of choice for drug development projects
● Success Rate: 100% guaranteed germline transmission (GLT)
● Turnaround Time: Founders as fast as 6-8 months
● Large Fragment Knockin: Insert fragments up to 300 kb
● Flexible Floxing: No size limitations for your floxed region
● 100% Money-Back Guarantee
CRISPR-Pro Gene Targeting Services
Cyagen's CRISPR-based gene targeting services can generate conditional knockout models - in as fast as 3 months.
Advantages of CRISPR Mouse Model Generation
● Large fragment: Fragments up to 15kb knockin and 500kb knockout
● Precise: Express your target gene accurately and precisely
● High efficiency: Improved HDR efficiency by using our unique fertilized mouse eggs process
● Guarantee: 100% money-back guarantee.
Cyagen Knockout Catalog Models
Cyagen Knockout Catalog Models repository provides a searchable listing of over 16k KO and cKO/floxed mouse strains with 100% pure B6 background, delivering affordable and ready-to-use live KO/cKO models to both research institutions and pharmaceutical corporations in as few as 3 months.
>> Search Your Gene of Interest
>> Weekly Flash Sale - KO/cKO Live Mice, Start from $999
Animal Model Supporting Services
Cyagen has established a range of supporting services, such as animal breeding, embryo/sperm cryopreservation, phenotype analysis services. Outsourcing these services to Cyagen is a more cost-effective way of conducting your research. All these services are performed by experienced specialists following standardized procedures, so we can guarantee delivery of high quality services and data acquisition with unbeatable price and turnaround.
● Phenotype Analysis Services
● Breeding Service
● Cryopreservation Service
● BAC Modification (Recombineering)
Contact us with your project details to request your complimentary model generation project consultation, strategy, and quote.
We will respond to you in 1-2 business days.