Cryopreservation Service

Cryoperservation of embryos and/or sperm is an ideal solution to maintain a live animal colony for strains that are rarely used. This backup can save significant space and prevent loss due to disease or in case an issue arises within your animal facility, providing an alternative to manage the colonies that will be used for research. Cyagen can back up your live animal colony by our embryo and sperm cryopreservation services, which can not only protect your valuable strains but also reduce your cost in the long run. We also can produce live animals for you in eight to twelve weeks once your cryopreserved stock is preserved at Cyagen.

◆ Cryopreservation Service

1. Embryo Cryoperservation

Embryo cryopreservation allows you to safeguard valuable research strains against loss caused by disease, breeding cessation or problems within your facility. With Cyagen's embryo cryopreservation service, you can protect your valuable strains with reduced cost in the long term. Procedures for embryo cryopreservation include superovulation of embryo donors (generally, female mice aged 3-4 weeks of the same background as the embryo donors.), mating of superovulated females to male mice, collection of two-cell embryos, cryopreservation of two-cell embryos, quality control throughout cryopreservation process and storage in liquid nitrogen.

 

2. Sperm Cryoperservation

Sperm cryopreservation creates a cost-effective and safety way to protect live animal colonies. Cyagen offers sperm cryopreservation service to back up your live animal colonies. Procedures for sperm cryopreservation include euthanizing 2 male mice from each line to be cryopreserved, collection and cryopreservation of sperm, quality control throughout cryopreservation process and storage in liquid nitrogen in liquid nitrogen.

 

3. Price and Requirements

Service	Description	Requirements	Deliverables	Price
Embryo Cryopreservation	For mouse strains	4 to 8 males(with genetic background) aged 6-8 weeks	At least 200 two-cell embroys/line	$1900/line (includes three years cryostorage) $100/line/year for extra years of storage
Sperm Cryopreservation	For mouse  strains	At least 2 males aged 8-16 weeks	4-5 vials of sperm/line	$1500/line (includes three years cryostorage) $100/line/year for extra years of storage

Note: The price above applies for the strains stocked at Cyagen. For others, please inquire about availability and pricing.

◆ Cryorecovery Service

Once your cryopreserved stock is preserved, we can produce animal lines for you in as little as eight weeks.

 

Service	Description						Deliverables	Price
Embryo Reconstitution	For mouse strains						Minimum 2 heterogeneous animals within 8-12 weeks	$1500/line
Sperm Reconstitution	For mouse strains						Minimum 2 heterogeneous animals within 8-12 weeks	$1500/line

Note: The price above applies for the strains cryopreserved and stocked at Cyagen. For others, please inquire about availability and pricing.

Why work with Cyagen?

• ● 14+ years' experience in custom animal model generation
• ● 78,000+ animal models generated and delivered worldwide
• ● 4,700+ citations in SCI journals for our services
• ● 3,000+ university and company partners
• ● 100% money-back guarantee option available for most services
• ● AAALAC-Accredited and OLAW Assured