It is widely known that single-nucleotide polymorphisms (SNPs) are primary factors that result in population diversity and clinical manifestations of disease – playing an essential role in personalized medicine. After researchers obtain clinical samples from the cohort(s), a large number of SNP candidate loci can be generated through second-generation sequencing and genome-wide association (GWA) analysis. However, in vivo research is necessary to verify the relationship between the SNP loci and clinical manifestations. If these SNPs are found in mice, point mutation mouse models can provide valuable information for clinical research.
Advance your SNP studies with the point mutation mouse model data bank independently developed by Cyagen, which can be used to quickly identify mouse mutation site(s) corresponding to the human SNP candidate loci and generate a mutation mouse model strategy. In order to further clinical research, precision medicine, and new drug research on this topic, we are hereby providing up to 15% off point mutation mice!
|Service Name||Number of Projects||Promotion Price (USD/Strain)||Promotional Savings||Model Ordering|
|CRISPR-Pro Point Mutation Mice||1-2 Projects||$13,000||Save up to $3000|
|CRISPR-Pro Point Mutation Mice||≥3 Projects||$12,000||Save up to $10000|
|CRISPR-Pro Point Mutation Mice||≥5 Projects||$11,250||Save over $16000+|
Cyagen offers rapid point mutation mouse model generation via CRISPR/Cas9-mediated genome editing technology by co-injecting nuclease and oligo donors carrying the desired mutation and homology arms. In this case, the Calca gene was chosen as a target, and guide RNAs (gRNAs) were designed. The gRNA, the donor oligo containing mutation and synonymous mutation (c.1138_1139 [TG to CC]), and Cas9 were co-injected into fertilized mouse eggs to generate targeted point mutation knockin offspring.
Diagram 1. The schematic describes the first stage in developing a point mutation mouse model via CRISPR.
Figure 1. PCR genotype screening of the F1 mice. Agarose gel electrophoresis of PCR results in F1 mice (#2, 4, 5, 8, and others) with site-specific gene knock-in.
The PCR product will be used for sequencing confirmation.
Figure 2. Representative illustration sequence analyses of F1 mice confirm F1 animals 2, 4, 5, and 8 with c.1138_1139 (TG to CC) mutation.
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Promotion for Conditional Knockout Mice
Conditional knockout mice play a vital role in biomedical advancements across many research areas which is essential for understanding human diseases and accelerating drug development. For a limited time, get your conditional knockout (cKO) mice for only $8,000 from our selection of Cyagen AI Knockout Mouse Model eBank. Take advantage of this promotion before it ends!