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TurboKnockout® - the Gold Standard of Genome Editing BAC-targeting Large Fragment Humanized Mice Ideal Drug Discovery Solution!

Although CRISPR/Cas9 technology is now a common technology for constructing gene-edited mice, it remains difficult to construct large-fragment gene knock-in (LFKI) or humanized mice using this approach. The possibility of off-target effects and patent disputes related to CRISPR/Cas9 technology present additional risks for pharmaceutical companies.

To solve the difficulties described above, it is recommended to use Cyagen's TurboKnockout® gene-editing technology. Based on traditional embryonic stem (ES) cell targeting techniques, TurboKnockout® provides accurate gene modifications, stable integration, no off-target risks, and can achieve LFKI (up to 300kb) through bacterial artificial chromosome (BAC) recombination. Importantly, the project cycle can be significantly shortened through multi-step BAC reorganization at the ES cell level. Compared to CRISPR/Cas9, TurboKnockout® is free of patent disputes and is the technique of choice for new drug development projects.

Bacterial artificial chromosome(BAC)is a low-copy vector that can hold more than 300kb of foreign DNA. Through either BAC homologous recombination or site-specific recombination at the ES cell level, the replacement of a mouse genome segment with corresponding human genome fragments can be achieved - which is the classic strategy of constructing humanized mouse models.For consultation and order, please call 86 20-31601779 or email

■ Promotion Period:August 1st 2020 - September 30th 2020

■ Eligibility:End clients in Asia Pacific region, excluding Japan.

Service Deliverables Promotion Price
TurboKnockout® ES cell targeting
Humanized Mice
≥4 Mice
Service Process
  • Targeting vector design & construction
  • Electroporation of ES cells to positive screening
  • Preparation of founder mice
  • F1 reproduction and screening
Service Advantages
Large fragment gene-editing
Limited by the capacity of DNA cloning vectors, foreign gene fragments integrated into genetically modified animals are usually less than 30kb. At this size limitation, key elements in regulating gene activity are often lost. BAC can accommodate foreign DNA of more than 300kb. By introducing larger genes and regulatory sequences through BAC modification, this allows greater control over the expression pattern of genes.
No off-target effects
TurboKnockout® is based on traditional ES cell targeting techniques; it can be used for complex gene knockouts for mice, providing models with accurate genetic modification, 100% germ line transmission, and no off-target effects.
No risk of patent infringement
Compared with CRISPR/Cas9 techniques, TurboKnockout® is free of patent disputes and is the technique of choice for new drug development projects.
Shorter turnaround
Founders as fast as 6 months thanks to two innovations that eliminate two generations of breeding: 1) super competent ES cell line generates 100% ESC-derived founders, avoiding the'chimera'phase; and 2) a self-removing Neo selection cassette that circumvents the need to breed to Flp deleter mice. In addition, multi-step BAC reorganization by TurboKnockout® at the ES cell level can shorten the production cycle.
Total publications citing Cyagen: 4,000
Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline.
Cell Metabolism PMID: 32004475 (2020)
Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation.
Nucleic Acids Res (2019) PMID: 31037292
Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses.
Immunity PMID: 30770248 (2019)
Human CPA1 mutation causes digestive enzyme misfolding and chronic pancreatitis in mice.
Gut 68: 301-312 (2019)
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