■ Promotion Period：August 1st 2020 - September 30th 2020
■ Eligibility：End clients in Asia Pacific region, excluding Japan.
| Service | Deliverables | Promotion Price |
|---|---|---|
| TurboKnockout® ES cell targeting Humanized Mice |
≥4 Mice |
Although CRISPR/Cas9 technology is now a common technology for constructing gene-edited mice, it remains difficult to construct large-fragment gene knock-in (LFKI) or humanized mice using this approach. The possibility of off-target effects and patent disputes related to CRISPR/Cas9 technology present additional risks for pharmaceutical companies.
To solve the difficulties described above, it is recommended to use Cyagen's TurboKnockout® gene-editing technology. Based on traditional embryonic stem (ES) cell targeting techniques, TurboKnockout® provides accurate gene modifications, stable integration, no off-target risks, and can achieve LFKI (up to 300kb) through bacterial artificial chromosome (BAC) recombination. Importantly, the project cycle can be significantly shortened through multi-step BAC reorganization at the ES cell level. Compared to CRISPR/Cas9, TurboKnockout® is free of patent disputes and is the technique of choice for new drug development projects.
Bacterial artificial chromosome（BAC）is a low-copy vector that can hold more than 300kb of foreign DNA. Through either BAC homologous recombination or site-specific recombination at the ES cell level, the replacement of a mouse genome segment with corresponding human genome fragments can be achieved - which is the classic strategy of constructing humanized mouse models.For consultation and order, please call 86 20-31601779 or email service-apac@cyagen.com.
■ Promotion Period：August 1st 2020 - September 30th 2020
■ Eligibility：End clients in Asia Pacific region, excluding Japan.
| Service | Deliverables | Promotion Price |
|---|---|---|
| TurboKnockout® ES cell targeting Humanized Mice |
≥4 Mice |
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Large fragment gene-editing Limited by the capacity of DNA cloning vectors, foreign gene fragments integrated into genetically modified animals are usually less than 30kb. At this size limitation, key elements in regulating gene activity are often lost. BAC can accommodate foreign DNA of more than 300kb. By introducing larger genes and regulatory sequences through BAC modification, this allows greater control over the expression pattern of genes. |
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No off-target effects TurboKnockout® is based on traditional ES cell targeting techniques; it can be used for complex gene knockouts for mice, providing models with accurate genetic modification, 100% germ line transmission, and no off-target effects. |
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No risk of patent infringement Compared with CRISPR/Cas9 techniques, TurboKnockout® is free of patent disputes and is the technique of choice for new drug development projects. |
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Shorter turnaround Founders as fast as 6 months thanks to two innovations that eliminate two generations of breeding: 1) super competent ES cell line generates 100% ESC-derived founders, avoiding the'chimera'phase; and 2) a self-removing Neo selection cassette that circumvents the need to breed to Flp deleter mice. In addition, multi-step BAC reorganization by TurboKnockout® at the ES cell level can shorten the production cycle. |
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