The Cre/lox recombination technique enables the generation of tissue-specific or inducible knockouts with a high level of control over the spatial and temporal expression of genes.

CRISPR Capabilities for Rat Models:

● Standard Use - Generate tissue-specific or inducible knockouts, such as in the KO-first conditional allele strategy

● Reporter Strains - Visually confirm locations of Cre recombinase activity with loxP-flanked fluorescent protein sequences

Cyagen Cre Rat Model Platform

One of the major constraints faced by researchers in developing conditional knockout (cKO) or conditional knockin (cKI) rat models is the lack of Cre lines specific to the target of interest. Given the higher facility fees associated with rats, the Creation of all strains necessary to achieve a Cre-lox rat strain can quickly become cost- or space-prohibitive.

To address this issue, Cyagen is building a repository of Cre models, including rat lines, available to researchers worldwide.

Rat Cre Lines Currently Under Development:

Still looking for your Cre line? Cyagen can provide pricing accommodations to researchers interested in Cre lines not currently available in our listings. Contact us to inquire about your conditional model needs and see how we can help develop a custom Cre strain to support your field of study.

Fill in the application form for a Free Cre Rat Trial - Cyagen support your field of study.

The construction time of viral vectors is always much shorter than that required for animal reproduction, especially to obtain the numbers needed for experiments. Cre rats need to be crossed with LoxP rats for one or two generations to obtain conditional genetically modified animals, but the probability of leakage and low efficiency of Cre enzymes may also affect progress of model development. Therefore, the most reliable strategy at present is to use the AAV virus to perform tissue-specific operations on existing LoxP rats while developing Cre and LoxP rats. Through this method, the phenotype of animals can be studied and verified more quickly, allowing early determinations for experimental follow-up strategies, which can greatly save the time on research projects.

The specific expression produced by AAV injection goes beyond just serving as a way to save time. Due to the uncertainty of isolated in vivo experiments in life science research and the inCreasingly abundant research methods developed to date, cross-validation with multiple methods can support your research results and improve the quality of your papers.