Knockout Mouse Catalog | Cyagen APAC
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Get Free Cre Driver Rat Strains Now With Conditional Rat Model Toolkit
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Rats are physiologically, morphologically, and genetically closer to humans than mice, which makes rats ideal models for biomedical and clinical studies. Its larger body and organ size facilitate multiple samplings, in vivo electrophysiology, as well as neurosurgical and neuroimaging procedures. Rat models are widely used in toxicology, teratology, endocrinology, oncology, neurology, experimental gerontology, cardiovascular research, dental research, and experimental parasitology.

Cyagen has made significant advancements across most aspects of the rat model production process, including ovulation promotion and improved fertilized egg injection techniques. Beyond optimizing our production efficiency, such improvements have facilitated the Creation of more complex genetically modified rat models than previously feasible – bringing the generation costs for rat models closer to that of mouse models. Cyagen now providing a variety of gene-editing rats services such as gene knockout (KO), gene knockin (KI), point mutation, and various strains options - SD, Wistar, Long Evans, F344, Brown Norway, etc. For consultation and order, please call 86 20-31601779 or email

Development of Conditional Models Using Cre/lox

The Cre/lox recombination technique enables the generation of tissue-specific or inducible knockouts with a high level of control over the spatial and temporal expression of genes.

CRISPR Capabilities for Rat Models:

● Standard Use - Generate tissue-specific or inducible knockouts, such as in the KO-first conditional allele strategy

● Transgene Switch -

a. Transgene Activation: A loxP-flanked “stop” sequence between the promoter and the coding sequence blocks expression (until bred with a Cre strain)

b. Inducible Knockout: A loxP-flanked transgene sequence may be removed upon breeding with a Cre strain

● Reporter Strains - Visually confirm locations of Cre recombinase activity with loxP-flanked fluorescent protein sequences

Cyagen Cre Rat Model Platform

One of the major constraints faced by researchers in developing conditional knockout (cKO) or conditional knockin (cKI) rat models is the lack of Cre lines specific to the target of interest. Given the higher facility fees associated with rats, the Creation of all strains necessary to achieve a Cre-lox rat strain can quickly become cost- or space-prohibitive.

To address this issue, Cyagen is building a repository of Cre models, including rat lines, available to researchers worldwide.

Rat Cre Lines Currently Under Development:

alb (Cre Ert2) myh6 (Cre Ert2) slc6a3 (Cre Ert2) camk2a (Cre Ert2) nphs2 (Cre Ert2)
alb (Cre) myh6 (Cre) slc6a3 (Cre) camk2a (Cre) nphs2 (Cre)
gfap (Cre) gfap (Cre Ert2) nes (Cre) nes (Cre Ert2)

Still looking for your Cre line? Cyagen can provide pricing accommodations to researchers interested in Cre lines not currently available in our listings. Contact us to inquire about your conditional model needs and see how we can help develop a custom Cre strain to support your field of study.

Fill in the application form for a Free Cre Rat Trial - Cyagen support your field of study.

AAV Virus – Solution for Cre Rats Construction

The construction time of viral vectors is always much shorter than that required for animal reproduction, especially to obtain the numbers needed for experiments. Cre rats need to be crossed with LoxP rats for one or two generations to obtain conditional genetically modified animals, but the probability of leakage and low efficiency of Cre enzymes may also affect progress of model development. Therefore, the most reliable strategy at present is to use the AAV virus to perform tissue-specific operations on existing LoxP rats while developing Cre and LoxP rats. Through this method, the phenotype of animals can be studied and verified more quickly, allowing early determinations for experimental follow-up strategies, which can greatly save the time on research projects.

The specific expression produced by AAV injection goes beyond just serving as a way to save time. Due to the uncertainty of isolated in vivo experiments in life science research and the inCreasingly abundant research methods developed to date, cross-validation with multiple methods can support your research results and improve the quality of your papers.

DPYSL2 Is a Novel Regulator for Neural Stem Cell Differentiation in Rats: Revealed by Panax Notoginseng Saponin Administration.
Stem Cell Research & Therapy (2020) PMID: 32299503
A xenograft and cell line model of SDH-deficient pheochromocytoma derived from Sdhb+/- rats.
Endocr Relat Cancer 2020 (2020) PMID: 32252027
Mutation in NPPA causes atrial fibrillation by activating inflammation and cardiac fibrosis in a knock-in rat model.
Faseb Journal (2019) PMID: 31034774
Hypoglycemic Effects of Glimepiride in Sulfonylurea Receptor Deficient Rat.
British Journal of Pharmacology (2018) PMID: 30471094

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