To verify the expression of conditional knockin (cKI) mice under normal conditions, the Flox knock-in mice needs to mate with a Cre line to remove the Stop-signal, generating mice that should express the knock-in across the tissues (specified by Cre). If the resulting expression is not as expected, the model will need to be reconstructed - which takes significant time, effort, and resources. The TurboKnockout® gene targeting technique from Cyagen can provide a rapid warning mechanism for early expression verification. When constructing the vector, tag proteins such as Flag or eGFP/mCherry are inserted into the over-expressed genes. After obtaining the ES clones with correct targeting, blastocyst injection will be performed, and ES cells will be subjected to Western Blot to verify the expression of the tag protein. Moreover, compared with CRISPR/Cas9-based techniques, TurboKnockout® is free of patent disputes - making it the technique of choice for new drug development projects. For consultation and order, Please call 86 20-31601779 or email service-apac@cyagen.com.
| Service | Deliverables | Turnaround Time | Promotion Price(USD/Strain) |
|---|---|---|---|
| TurboKnockout® Conditional Knockin Mice (Rosa26 locus) +WB tag verification at cell level | 4 TurboKnockout® Mice | 6-8 months | $20,000 |
| TurboKnockout® Conditional Knockin Mice (Rosa26 locus) + Breeding + WB tag verification at cell level | 4 Tissue-specific expressing heterozygotes + 4 Flox heterozygotes | 7-9 months | $23,000 |
| TurboKnockout® Conditional Knockout Mice + Breeding | 2 pairs of cKO homozygotes + 2 pairs of Flox homozygotes | 11 months | $26,000 |
service-apac@cyagen.com
86 20-31601779
We will respond to you in 1-2 business days
We provide the additional services such as Western Blot tag verification at the cell level, such early expression verification is more cost-effective.
TurboKnockout® is based on traditional ES cell targeting techniques; it can be used for complex gene knockouts, providing models with accurate genetic modification, 100% germ line transmission, and no off-target effects.
Compared with CRISPR/Cas9 techniques, TurboKnockout® is free of patent disputes and is the technique of choice for new drug development projects.
TurboKnockout® gene targeting technology can provide a rapid warning mechanism for expression verification. When constructing the vector, tag proteins such as Flag or eGFP / mCherry are inserted into the over-expressed genes. After obtaining the ES clones with correct targeting, blastocyst injection will be performed, and ES cells will be subjected to Western Blot verification to detect the expression of the tag protein. Double verification can provide the best assurance for achieving the desired expression.
Founders as fast as 6 months thanks to two innovations that eliminate two generations of breeding: 1) super competent ES cell line generates 100% ESC-derived founders, avoiding the ‘chimera’ phase; and 2) a self-removing Neo selection cassette that circumvents the need to breed to Flp deleter mice.
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