Using CRISPR to Develop Knockout Mouse Models for Research
Engineered nuclease-mediated genome editing, especially CRISPR, has emerged as a promising technology which can serve as an alternative to the conventional, embryonic stem (ES) cell homologous recombination-based generation of animal models.
CRISPR, which stands for “Clustered regularly interspaced short palindromic repeats,” works alongside Cas proteins to form the CRISPR-Cas complex – a biological technology originally derived from a prokaryotic adaptive immunity system. To show how this process works, our video introduces the individual components of a CRISPR-Cas complex using the SHANK3 gene as an example.
Some people may think that gene knockout (KO) models are mostly developed using traditional embryonic stem (ES) cell technology, however, CRISPR has become widely used to generate these KO animal models due to a faster turnaround time, lower cost, and higher targeting efficiency has led to its widespread use in the industry. Using the SHANK3 gene as an example, this video shows you how scientists use CRISPR-Cas9 to generate knockouts in mice as well as other animals.
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