Dr. Steve Yu
Senior Scientist of Cyagen
(Note: This article is translated from the original writing in Chinese)
The mouse model has become an irreplaceable model organism for biomedical research, which includes studying gene function and genetic factors of pathogenesis, establishing humanized disease models, and evaluating the safety and effectiveness of drugs under development. The rapidly growing branch of biomedical research known as translational medicine is aimed at the practical problems from clinical patients. A combination of molecular, cellular, and animal models in related basic and preclinical studies can help address the problems of translational research. Therefore, a major strategy of translational medical research is to combine the basic laboratory studies with the preclinical animal experimental research and rapidly move into clinical application.
1. How to Use the Appropriate Mouse Model to Publish High-Impact Articles?
“In Vivo AAV-CRISPR/Cas9-mediated Gene Editing Ameliorates Atherosclerosis in Familial Hypercholesterolemia”published in the well-known journal, Circulation, this year. Using this article, I will further explain how to apply the gene editing mouse model to carry out research about the relationship between genes and diseases and how to use gene editing to create a mouse model which will adequately evaluate the effectiveness of specific gene therapies.
2. Humanized Mouse Models – Introduction and Applications
The term‘humanized mouse’was referred to mouse models which included the human immune system (HIS) - which was achieved by transplanting human cells or tissues into immune-deficient mice. However, the term has grown to include custom mouse models genetically engineered to express human gene products, the introduction of human intestinal microbiota into sterile mice, among other methods that may provide in vivo context for human pathologies and physiology. Thus, a humanized mouse may describe any model in which human cell tissue, genes, or intestinal microorganisms are introduced through xenotransplantation or gene editing technology.
There are numerous types of humanized mouse models to choose from. Every model comes with its own unique advantages and challenges across various research foci. Whether you are interested in genetically modified or immunodeficient models for xenotransplantation, our experts are available to provide free consultations and quotes. Contact us with your project details to receive more information within 48 hours.
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During 14 years of development, Cyagen has served tens of thousands of scientists around the world. Cyagen’s products and technologies have been directly applied to more than 4,300 academic papers across major journals- including Cell, Nature, and Science. In addition to providing customized services for knockout (KO), knock-in (KI) and conditional knockout (cKO) models, Cyagen also has a professional surgical disease model team, which can provide a variety of complex and sophisticated surgical disease small animal models. Cyagen’s drug screening and evaluation mouse platform can be used to search for the immunodeficient mice introduced from European and American industry leaders and the humanized mice for cardiovascular and Alzheimer's disease research. Cyagen’s international standardized sterile mouse technology platform can provide various products and services based on aseptic animal models, such as aseptic mice, aseptic animal customization services, and microbial flora transplantation services. In combination with Cyagen’s mature and stable gene editing mouse platform, you can study the specific interaction mechanisms between bacteria and genes.
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