Description:
Embryonic stem cells (ESCs), derived from the inner cell mass (ICM) of blastocyst-stage embryos, are pluripotent and have a virtually unlimited capacity of self-renewal. They can differentiate into all cell types comprised of all three embryonic germ layers (ectoderm, mesoderm and endoderm). The formation of embryoid bodies (EBs) is a principal step in the differentiation of ESCs. When maintained in the EB formation medium and in the absence of MEF feeder layers, ES cells differentiate spontaneously and form three-dimensional aggregates. This structure facilitates multicellular interactions in which cell-cell contact occurs and gap junctions are established.
Highlights:
- Highly effective in inducing human and mouse embryonic stem cells to form embryoid bodies (EB).
- Batch-to-batch consistency.
Kit Components:
|
Embryoid Body (EB) Formation Basal Medium |
435 mL |
|
Embryoid Body (EB) Formation- Qualified Fetal Bovine Serum |
50 mL |
| Penicillin-Streptomycin | 5 mL |
| Glutamine | 5 mL |
| Non-Essential Amino Acid | 5 mL |
| 2-Mercaptoethanol | 500 μl |
Stability/Storage:
- All products should be stored in the dark.
- Embryoid Body Formation Basal Medium is stable at 2-8oC for up to one year; other components are stable at -20oC for up to two years.
- Products should be discarded beyond the labeled expiration date.
- Once prepared, the fully supplemented (complete) medium can be stored for up to one month when stored in the dark at 2-8oC.
- For optimal performance, repeated warm-cooling and freeze-thawing should be avoided.
Quality Control:
Tested for perfomance on EB formation. The standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
3. Osmolality
4. Endotoxin
Intended Use:
Intended for non-human laboratory research use only.
