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Non-Essential Amino Acid (NEAA) Cell Culture Supplement

Non-Essential Amino Acid (NEAA) Cell Culture Supplement NEAA-10201-50
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NEAA Cell Culture Supplement is a 100× stock solution containing 7 kinds of non-essential amino acids. It is used as a supplement to cell culture media for optimized cell growth.

Most cells can synthesize non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration level are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

The seven amino acids are resolved in pH 1.5~1.7 HCl solution.

 Amino Acids  Concentration (mg/l)
 Glycine  750
 L-Alanine  890
 L-Asparagine  1320
 L-Aspartic acid  1330
 L-Glutamic Acid  1470
 L-Proline  1150
 L-Serine  1050


- Store at 2-8°C for up to one year.
- Avoid repeated warm-cooling and freeze-thawing for optimal performance

Quality Control:
Standard evaluation includes:

1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
3. Osmolality
4. Endotoxin

Instruction for Use:
1 mL NEAA Cell Culture Supplement is added into 99mL cell growth medium for cell culture.