Knockout Mouse Catalog | Cyagen APAC

Wound Healing (Scratch Test)

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The in vitro wound/scratch assay is a convenient and economical method for studying cell migration/tumor invasion. The experimental principal is to allow cells to grow to a single layer of fusion, then artificially create a blank area (also called a "wound") on these cells. The cells on the edge of the space will gradually enter the blank area to close the wound.


Compared to other methods, the in vitro wound/scratch assay simulates the process of in vivo cell migration to a degree, and is the simplest among in vitro testing methods for studying cell migration.


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