APOE Knockout Mouse

Genetic Background: C57BL/6

Strain Origin:

The model was created by targeting the ApoE gene in E14TG2a ES cells and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed for ten generations (N10) and intercrossed to homozygosity, and then backcrossed one additional generation (N11). The line was derived by embryo transfer and is maintained by backcrossing with homozygous mice.

Genetics:

Wild type for Nnt mutation. This line carries the Gpi1a allele and the ES1a and ES1b alleles in contrast to the C57BL/6N inbred which is Gpi1b and ES1a

Strain Description:

• ■   The mouse endogenous apolipoprotein E (APOE) gene is disrupted.
• ■   Homozygous ApoE knockout mice are devoid of apoE protein.
• ■   Mice develop normally, but exhibit five times normal serum plasma cholesterol and spontaneous atherosclerotic lesions.
• ■   Studies indicate a role for ApoE in immune system regulation, nerve regeneration, and muscle differentiation.

Research& Application:

Useful in studying the role of ApoE in lipid metabolism, atherogenesis, and nerve injury and to investigate intervention therapies that modify the atherogenic process.

Health Standard: SPF