Genetic Background: C57BL/6, SJL Mixed Background
This model was created by microinjecting the human APP695 gene containing the double mutation K670N, M671L into B6SJLF2 zygotes using a hamster prion protein cosmid vector. The resultant mice from Founder Line 2576 were backcrossed to C57BL/6. Hemizygous males were backcrossed with C57BL/6 females for derivation by embryo transfer. The colony is maintained by mating hemizygous male mice with B6SJLF1 female mice.
Genetics: Wild type for Nnt mutation; carries Pde6brd1 mutation
- ■ Carries a transgene coding for the 695-amino acid isoform of β-amyloid precursor protein (APP) carrying the Swedish mutation.
- ■ Expresses high concentrations of the mutant Aβ, develops significant amyloid plaques and displays memory deficits.
- ■ Premature mortality is an expected phenotype of this line, with mortality of 20% anticipated, particularly in males. Please take this into consideration when deciding upon order size to retain the needed experimental group size.
- ■ It is recommended that customers purchase mice between 10 and 12 weeks of age. Due to a phenotype of sudden death at young ages, it’s hard to guarantee the shipment of mice 9 weeks of age or younger.
- ■ Between 7 to 12 weeks of age males become aggressive and begin to fight. We highly recommend housing APPSWE male mice one per cage.
- ■ Pink eyed animals, associated with certain coat colors, and the Pde6brd1 retinal degeneration mutation can cause light sensitivity and/or blindness in some animals. This may impact the results of behavioral testing. Upon request, animals can be screened for eye color, coat color, and/or rd1 homozygosity for an additional fee.
- ■ Barbering is common in this strain, particularly in older females, and is usually caused by over-grooming from a cagemate. Barbered animals display alopecia (hair loss) around the eyes, nose, head or back, which can range from mild hair loss to large areas of missing hair. Skin is typically not affected, and no medical treatment is indicated for hair loss caused by barbering. Because of the high incidence of barbering in this strain, animals with mild to moderate barbering may be included in shipments.
Useful for the study of APP expression, amyloid plaque formation, neuronal decline and memory loss associated with Alzheimer's disease (AD) as well as the study of drugs designed for treatment or prevention of Alzheimer's disease
Health Standard: SPF