Many researchers have combined the genetic modification model with diet or drug induction to produce a composite model, which makes the phenotype and pathogenesis of the model closer to that of human NAFLD. Composite models can more accurately reflect the progression of the disease development - from simple fatty liver to NASH, and progression to liver fibrosis.
The Most Commonly Used Composite Models: ob/ob mice + methionine-choline deficient (MCD) diet; db/db mice + methionine choline deficient (MCD) diet, Zucker rats + methionine choline deficient (MCD) diet/high fat diet.
The 'db/db mouse + MCD diet' model is more severe than the 'ob/ob mouse + MCD diet' model in both inflammation and fibrosis around the cells, and also demonstrates a significantly shortened induction period - which has led to predominate use of the 'db/db mouse + MCD diet' model.
Detection Items: body weight, food intake, serum biochemical test (alanine aminotransferase, aspartate aminotransferase, serum total cholesterol, triglyceride content, etc.), pathological analysis of liver tissue (liver tissue H & E staining, oil red).