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Diabetes Mellitus (DM) has become the top third major chronic disease threatening human health after cancer and cardiovascular disease. This chronic disease is diagnosed when the body cannot secrete sufficient insulin or the insulin produced does not work effectively, resulting in increased glucose levels. Type 2 diabetes mellitus (T2DM) patients account for about 90% of all diabetic patients, and most patients with T2DM are experience insulin resistance. The T2DM mouse model can be used in exploring the hypoglycemic effect of drugs on type 2 diabetic mice, which can be prepared by methods such as diet, chemical drugs, surgery, and gene editing.
Construction Strategy: T2DM mouse model was constructed with a high-sugar and high-fat diet fed combined with low-dose intraperitoneal injection of streptozotocin (STZ).
Background: C57BL/6J
Weight: Greater than 18g, the average value is preferably about 20g.
Experimental groups: There are five experimental groups - model control group, positive control group, low-dose group, medium-dose group, and high-dose group.
Testing items: Body weight, food intake, glucose tolerance test (GTT), insulin tolerance test (ITT), fasting blood glucose test, random blood glucose test, serum biochemical test or kit method (ELISA test kit), physiological cage test (urine and feces collection), metabolic cage detection (indicators such as oxygen consumption, heat production, activity, etc.), tissue and organ collection and pathology analysis (H&E, Sirius red, Masson, Oil red, IHC, etc.), etc.
Principles: Continuous high-fat diet (HFD) induced obesity mouse, which can be used for obesity and type 2 diabetes research.
Background: C57BL/6J
Weight: Greater than 18g, the average value is preferably about 20g.
Experimental groups: There are five experimental groups - model control group, positive control group, low-dose group, medium-dose group, and high-dose group.
Test items: Body weight, food intake, glucose tolerance test (GTT), insulin tolerance test (ITT), fasting blood glucose test, random blood glucose test, serum biochemical test or kit method (ELISA test kit), physiological cage test (urine and feces collection), metabolic cage detection (indicators such as oxygen consumption, heat production, activity, etc.), tissue and organ collection and pathology analysis (H&E, Sirius red, Masson, Oil red, IHC, etc.), etc.